Recombinant Human Collagen alpha-1(III) chain (COL3A1) 重组蛋白-武汉华美生物 (2024)

中文名称：
Recombinant Human Collagen alpha-1(III) chain (COL3A1)
货号：

CSB-MP005740HU(A4)d7
规格：

￥1608
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

COL3A1
Uniprot No.：

P02461
种属：

Homo sapiens (Human)
蛋白长度：

Full Length of Mature Protein
See Also
Decreased Collagen Production in Chronologically Aged Skin: Roles of Age-Dependent Alteration in Fibroblast Function and Defective Mechanical Stimulation ASpdb: Protein Annotation DataBase - Search Specific Collagen Peptides Improve Bone Mineral Density and Bone Markers in Postmenopausal Women—A Randomized Controlled Study
来源：
Mammalian cell
分子量：

98.8 kDa
表达区域：

154-1221aa
氨基酸序列
QYDSYDVKSGVAVGGLAGYPGPAGPPGPPGPPGTSGHPGSPGSPGYQGPPGEPGQAGPSGPPGPPGAIGPSGPAGKDGESGRPGRPGERGLPGPPGIKGPAGIPGFPGMKGHRGFDGRNGEKGETGAPGLKGENGLPGENGAPGPMGPRGAPGERGRPGLPGAAGARGNDGARGSDGQPGPPGPPGTAGFPGSPGAKGEVGPAGSPGSNGAPGQRGEPGPQGHAGAQGPPGPPGINGSPGGKGEMGPAGIPGAPGLMGARGPPGPAGANGAPGLRGGAGEPGKNGAKGEPGPRGERGEAGIPGVPGAKGEDGKDGSPGEPGANGLPGAAGERGAPGFRGPAGPNGIPGEKGPAGERGAPGPAGPRGAAGEPGRDGVPGGPGMRGMPGSPGGPGSDGKPGPPGSQGESGRPGPPGPSGPRGQPGVMGFPGPKGNDGAPGKNGERGGPGGPGPQGPPGKNGETGPQGPPGPTGPGGDKGDTGPPGPQGLQGLPGTGGPPGENGKPGEPGPKGDAGAPGAPGGKGDAGAPGERGPPGLAGAPGLRGGAGPPGPEGGKGAAGPPGPPGAAGTPGLQGMPGERGGLGSPGPKGDKGEPGGPGADGVPGKDGPRGPTGPIGPPGPAGQPGDKGEGGAPGLPGIAGPRGSPGERGETGPPGPAGFPGAPGQNGEPGGKGERGAPGEKGEGGPPGVAGPPGGSGPAGPPGPQGVKGERGSPGGPGAAGFPGARGLPGPPGSNGNPGPPGPSGSPGKDGPPGPAGNTGAPGSPGVSGPKGDAGQPGEKGSPGAQGPPGAPGPLGIAGITGARGLAGPPGMPGPRGSPGPQGVKGESGKPGANGLSGERGPPGPQGLPGLAGTAGEPGRDGNPGSDGLPGRDGSPGGKGDRGENGSPGAPGAPGHPGPPGPVGPAGKSGDRGESGPAGPAGAPGPAGSRGAPGPQGPRGDKGETGERGAAGIKGHRGFPGNPGAPGSPGPAGQQGAIGSPGPAGPRGPVGPSGPPGKDGTSGHPGPIGPPGPRGNRGERGSEGSPGHPGQPGPPGPPGAPGPCCGGVGAAAIAGIGGEKAGGFAPYYG
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：
C-terminal 10xHis-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
基因功能参考文献：
1. Elevated circulating PIIINP levels are associated with type 2 diabetes mellitus individuals with adipose tissue expansion and systemic proinflammatory profile suggestive for adipose tissue dysfunction. PMID: 29471595
2. Reduced expression of types I and III collagen and TIMP-1 as well as the increased expression of MMP-1 and MMP-8 in the anterior vaginal wall tissues play important roles in the onset of pelvic organ prolapse. PMID: 29263043
3. a variant of COL3A1 (rs3134646) is associated with the risk of developing colonic diverticulosis in white men, whereas rs1800255 (COL3A1) and rs1800012 (COL1A1) were not associated with this condition after adjusting for confounding factors. PMID: 29533249
4. Brain MRI in the affected siblings as well as in the two previously reported individuals with bi-allelic COL3A1 mutations showed a brain phenotype similar to that associated with mutations in GPR56. PMID: 28258187
5. Given the high specificity of the polymorphism at the rs1800255 locus of the COL3A1 gene, determined by the Sanger sequencing, it can be concluded that there is an association between this polymorphism and urinary incontinence and pelvic organ prolapse in women. PMID: 29376591
6. Case Report: novel missense COL3A1 mutation in vascular Ehlers-Danlos syndrome patient presenting with pulmonary complications and iliac arterial dissection. PMID: 29216800
7. Kaplan-Meir analysis of GSE7696 indicate that COL3A1 and SNAP91 correlated with survival. PMID: 27655637
8. provide a picture of the gene expression changes in vascular Ehlers-Danlos syndrome skin fibroblasts and highlight that dominant negative mutations in COL3A1 also affect post-translational modifications and deposition into the ECM of several structural proteins crucial to the integrity of soft connective tissues PMID: 29346445
9. We identified biallelic COL3A1 variants in two unrelated families. In a 3-year-old female with developmental delay the nonsense variant c.1282C>T, p.(Arg428*) was detected in combination the c.2057delC, p.(Pro686Leufs*105) frame shift variant. PMID: 28742248
10. In conclusion, based on serological collagen formation and degradation makers, penetrating Crohn's disease is associated with increased matrix metalloproteinase-9 mediated breakdown of type III collagen PMID: 28481042
11. Col3A may be a potential adjunct marker for both differentiating fibroadenoma from phyllodes tumor and assessing malignant potential in PTs. PMID: 27498063
12. Case Report: pathogenetic heterozygous COL3A1 mutation c.3140 G>A, p. Gly1047Asp in Ehlers-Danlos syndrome vascular type with different phenotypes in the same family. PMID: 28183226
13. von Willebrand factor A1 domain-collagen binding is independent of gain- or loss-of-function phenotype and under shear stress, platelet translocation pause times on collagen-bound A1A2A3 are either normal or shorter depending on whether A1 is concertedly bound with the A3 domain to collagen. PMID: 27889474
14. High serum procollagen type III N-terminal peptide expression is associated with non-alcoholic fatty liver disease. PMID: 27442361
15. The findings indicate the critical role of CatB in regulating the expression of collagens III and IV by fibroblasts via prolonging TLR2/NF-kappaB activation and oxidative stress. PMID: 27648120
16. High COL3A1 expression is associated with colorectal carcinoma. PMID: 26741506
17. novel missense mutation c.2176G>C in Chinese family with vascular Ehlers-Danlos syndrome PMID: 25420629
18. High serum Collagen Type III is associated with ovarian and breast cancer. PMID: 26406420
19. A novel missense mutation in COL3A1 was found in a young patient with cervical artery dissection as the single manifestation of Ehlers-Danlos syndrome. PMID: 26497932
20. the clinical phenotype of Ehlers-Danlos syndrome patients is influenced by the type of COL3A1 variant. PMID: 25758994
21. Report dysregulated expression of COL3A1 in disc degeneration. PMID: 25893343
22. TGFbeta target genes including TGFBI, BAMBI, COL3A1 and SERPINE1 are significantly increased in Diamond Blackfan Anemia induced pluripotent stem cells PMID: 26258650
23. The production and purification of rhCOL3A1 described in this study offer a new method for obtaining high level of rhCOL3A1 in relatively pure form, which is suitable for biomedical materials application. PMID: 25231012
24. These results suggest that let-7d may suppress renal cell carcinoma growth, metastasis, and tumor macrophage infiltration at least partially through targeting COL3A1 and CCL7. PMID: 25193015
25. compare reported phenotypes for patients with missense variants in the C-propeptide domain for other human collagen disorders including COL1A1 and COL1A2 (osteogenesis imperfecta). PMID: 25846194
26. miR-29a and miR-29b enhance cell migration and invasion in nasopharyngeal carcinoma progression by regulating SPARC and COL3A1 gene expression. PMID: 25786138
27. Data indicate that N-terminal propeptide of type III procollagen (PIIINP) is a highly effective means to evaluate left ventricular (LV) end-diastolic pressure (EDP) in patients with acute coronary syndrome (ACS). PMID: 25559610
28. The first main finding of this study was that the rare COL3A1 rs1800255 AA genotype was associated with increased risk of anterior cruciate ligament injuries in the Polish cohort but not the South African cohort. PMID: 25073002
29. During heart valve development, Krox20-mediated activation of fibrillar Col1a1 and Col3a1 genes is crucial to avoid postnatal degeneration of the aortic valve leaflets. PMID: 25344368
30. In familial AAA we found one pathogenic and segregating variant (COL3A1 p.Arg491X), one likely pathogenic and segregating (MYH11 p.Arg254Cys), and fifteen VUS. PMID: 26017485
31. rs1800255, COL3A1 2209 G>A polymorphism not associated with pelvic organ prolapse in Dutch population PMID: 24760181
32. Variants of the COL3A1 gene are associated with a risk of stroke recurrence and prognosis. PMID: 24664438
33. As a result of the translocations, COL3A1-PLAG1, the constitutively active promoter of the partner gene drives the ectopic expression of PLAG1. PMID: 24700772
34. Gal-1 decreased the expression of collagen genes COL3A1 and COL5A1 but increased the expression of fibronectin and laminin 5. PMID: 24503541
35. Arterial pathology in vascular Ehlers-Danlos syndrome individuals is related to the underlying COL3A1 mutation type. PMID: 24650746
36. Expression of COL3A1 mRNA encoding key fibrotic extracellular matrix molecules was down-regulated by pre-miRNA-29b. PMID: 24641356
37. The results point to the fact that the injury and/or mandible fracture increase the collagen type III metabolism and its dynamics depends on the type of the used bone fixation. PMID: 24421219
38. Case Report: Ehlers-Danlos syndrome IV due to a mutation in intron 14 of the COL3A1 gene leading to venous manifestations without affecting arterial vessels at clinical presentation. PMID: 23688910
39. Data suggest that cardiac fibrosis, as assessed by serum extracellular matrix protein biomarkers (including PIIINP, procollagen Type III-N-terminal peptide), develops early in hypertensive patients and is predictive of cardiovascular events or death. PMID: 22788708
40. analysis of collagen folding in Vascular Ehlers-Danlos syndrome mutations PMID: 23645670
41. COL3A1 rs1800255, COL6A1 rs35796750 and COL12A1 rs970547 were not significantly associated with sit-and-reach, straight leg raise or total shoulder rotation range of motion PMID: 23013106
42. This study indicates that SP, mediated via NK-1 R, increases collagen remodeling and leads to increased MMP3 mRNA and protein expression that is further enhanced by cyclic mechanical loading. PMID: 22836729
43. IL-17, IL-23 and PIIINP have an inverse correlation with vitamin D in their involvement in the immune response in patients with HCV-4-related liver diseases in Egypt PMID: 22851868
44. study found that allele A of SNP rs1800255 conferred a 1.71-fold increased risk for intracranial aneurysms (IAs) and results in an amino acid change of Ala698Thr, which led to a lower thermal stability of the peptide; results support the view that the functional variant of COL3A1 is genetic risk factors for IAs in the Chinese population PMID: 22241462
45. The minimum type III sequence necessary for cleavage by the MMP1 and MMP13 was 5 GXY triplets, including 4 residues before and 11 residues after the cleavage site (P4-P11'). PMID: 22573319
46. Alveolar macrophages exposed to SiO2 can induce elevated type III procollagen and collagen III expression levels in human lung fibroblasts. PMID: 21941774
47. Through alveolar macrophage mediation SiO2 can accelerate the expression of TIMP-1 and collagen III, and inhibit the expression of MMP-1 in human lung fibroblasts. PMID: 20039537
48. procollagen III N-terminal peptide has a role in HBeAg loss in patients with chronic hepatitis B during entecavir therapy PMID: 22019950
49. Disease-associated mutations prevent GPR56-collagen III interaction. PMID: 22238662
50. Data show that homozygous and compound heterozygous changes found in PLOD1 and SLC2A10 may confer autosomal recessive effects, and three MYH11, ACTA2 and COL3A1 heterozygous variants were considered as putative pathogenic gene alterations. PMID: 22001912
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相关疾病：

Ehlers-Danlos syndrome 3 (EDS3); Ehlers-Danlos syndrome 4 (EDS4)
亚细胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Fibrillar collagen family
数据库链接：

HGNC: 2201

OMIM: 120180

KEGG: hsa:1281

STRING: 9606.ENSP00000304408

UniGene: Hs.443625

Recombinant Human Collagen alpha-1(III) chain (COL3A1) 重组蛋白-武汉华美生物 (2024)

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